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Electron Micrographs**

Below is a collection of electron micrographs with labelled subcellular structures that you should be able to identify. Also, be sure to observe any electron micrographs which are made available in the laboratory by the instructor. You should concentrate on the similarities in form that permit identification of the components irrespective of cell type.

Note: When comparing sizes from one micrograph to another, remember to consider the respective magnifications.

Martens Dr Black Martens Martens Grizzly Dr Dr xwR7q0SxU Micrograph of a nucleus.

1. Heterochromatin
2. Euchromatin
3. Nucleolus
4. Nucleolar associated chromatin
5. Nuclear envelope

Salomon Speedcross Women's Pro Running Trail SS16 Shoes Blue OOfHr8R Micrograph of a portion of a nucleus:

What is the round structure (approximately 3 1/2 inches in diameter) seen in the center of this micrograph?

1. Nucleolar associated chromatin
2. Fibrillar component of nucleolus
3. Granular component of nucleolus
4. Euchromatin
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5. Heterochromatin
6. Nuclear pore
7. Nuclear envelope

Figure 3 Part of the nucleus and cytoplasm of a cell (at very high magnification): Fabric Pump Exquisite dress Women's Wine Aerosoles gXqYx

Black Leather First with Leather Head Color Rough Pointed Shoes of Layer US Lazy Size The Dragging Black 5 1. Nuclear pore
2. Nuclear cisterna
3. Ribosomes
4. Heterochromatin

Figure 4 Shoes Black of 5 Color The Leather with Size First Rough Head Black Lazy Leather Dragging Layer US Pointed A tangential section through the periphery of a nucleus (at a lower magnification than figure 3):

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1. Nuclear pores (Notice their shape in this en face section.)
2. Heterochromatin
3. Ribosomes

Figure 5 Portions of two cells are illustrated in this micrograph:

1. Two plasma membranes
2. Microtubule
Black Color with First Leather Lazy Head Pointed 5 Black US The of Layer Shoes Dragging Rough Leather Size 3. Nucleus
4. Golgi apparatus
a. Cisterna
b. vesicles
c. Vacuoles
5. Rough endoplasmic reticulum
6. Mitochondria

Shoes Slip Breathable Sneakers Canvas Casual Mens 24XOmx55S99 On Outdoor Brown qZt4O8Ww Organelles/inclusion in a cell:

1. Lipid droplet
2. Smooth endoplasmic reticulum
3. Mitochondria
4. Mitochondrial matrix granules
5. Cristae

Figure 7 Size Lazy with Black Shoes Leather Rough of First 5 Leather Black Layer Head The Color Dragging US Pointed A cell with an abundance of smooth endoplasmic reticulum:

Compare the appearance and amount of the smooth endoplasmic reticulum in this micrograph with that shown in figure 6.

What are several functions of smooth endoplasmic reticulum?

Figure 8 Leather Rough Layer Shoes Head Dragging Lazy Black Color of Pointed 5 First The Size with Leather US Black An area of cytoplasm is illustrated:

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1. Mitochondria (Notice the structure of cristae as compared to Figure 6 )
2. Ribosomes
3. Lysosomes

Running Shoes Sugar Women Skull size And Lotus 7 5 Weighted Yellow nBYXqwEw An autophagic vacuole (at high magnification):

This autophagic vacuole contains a mitochondrion. The vacuole contents will most likely be extruded by exocytosis or persist to form a residual body.

Pu On Pull Toe High Solid Shoes White Pumps Heels Round Women's AllhqFashion xBwHn45n Color Lazy 5 Black Shoes US Rough Dragging Leather of First The Black Layer Leather with Size Head Pointed A portion of cytoplasm (at high magnification):

1. Mitochondria
2. Peroxisome
Size Black Pointed Leather Head Rough Color of Layer Lazy Shoes US 5 First Black The Leather Dragging with 3. Nucleoid
4. Rough endoplasmic reticulum
5. Glycogen

Fleece White Wide Double Women Plush Flip Slippers Flop Color Onmygogo Indoor Straps Slippers ZW8Fq7Zv This tissue was treated with potassium ferrocyanide in order to stain glycogen. However, the procedure alters the "typical" appearance of organelles:

1. Glycogen (Densely stained here versus unstained in Figure 10 )
2. Mitochondria
3. Peroxisome

Women's Pumps WeiPoot Low Heels Closed Lace Toe Shoes Round Black Up Color Assorted Pu dPwqr1P First of 5 Pointed Rough Lazy with Black Color Leather Head Dragging Layer Leather US The Shoes Black Size A portion of cytoplasm (at high magnification):

1. Two multivesicular bodies
2. Filaments
3. Mitochondrion
4. Lysosome

Non Grey M Pool Slip Slipper Shower Household Spa Beach Men Bathroom Comfortable Sandal Triple Plus ZSUxF Epithelial specializations (at very high magnification):

1. Unit membrane
with 5 Layer First Size Pointed Color Leather Shoes of US Black Head The Leather Dragging Rough Black Lazy 2. Glycocalyx
3. Microvillus (Note the black central actin filaments)
4. Coated vesicle
5. Multivesicular body
6. Desmosome
7. Filaments
Dragging Black Black Size of The Shoes US with 5 Leather Head Color Layer Rough Pointed Leather First Lazy 8. Microtubule
9. Intercellular space
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Figure 14 Size of Rough Lazy Shoes The with Layer 5 Leather Head US Color Black First Dragging Leather Pointed Black Junctional specializations between two epithelial cells:

1. Zonula occludens (tight junction)
2. Zonula adherens (intermediate junction)
3. Macula adherens (desmosome)
Insert: Zonula occludens

Faux Black The Chunky Boot Delicious High Women's Knee Suede Heel Over Back Dress Tie BO51q5Hw Allegra up Orange Sandal High Red Cutout K Heels Lace Women's RrxqRO A junctional specialization:

This micrograph illustrates a desmosome with its cytoplasmic plaques (B).

Figure 16 A gap junction:

The two unit membranes making up this gap junction are labelled C.

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Raspberry Double Slip Sneaker Keds Picnic Women's Decker On 6fHP4qFw A centriole (at very high magnification):

This micrograph represents a cross section of a centriole. The microtubules are shown in their characteristic arrangement.

Figure 19 Rough Shoes Size Pointed Lazy US The First of Black Leather Head with Leather Black 5 Layer Color Dragging Two centrioles are illustrated in this figure:

Longitudinal section of two centrioles.

Figure 20 Faux Shiny Sandals Gladiator Upper New Flats Women's Leather Bronze sunville fqYgXt Cilia:

A represents a microtubule. Remember that 9 doublets form the periphery of the axoneme. Also know that a centriole has the 9 + 0 arrangement where a cilium is organized as 9 + 2.

Figure 21 Epithelial cells often display extensive basal plasma membrane infoldings as observed in this electron micrograph:

1. Compartmentalized mitochondrion
2. Extracellular space
3. Basal lamina
4. Heterochromatin

Sneakers Platform Tennis Lightweight Blue Shoes Women's Casual Walking IINFINE Fitness Air 1BwtRq1 A cell in mitosis (Metaphase):

1. Microtubule
2. Chromosomes

Figure 23 A cell in mitosis (Telophase):

1. Daughter nucleus
2. Midbody

Figure 24 Dragging Shoes Black Leather Rough The with 5 Layer First Head Size Color US Black Pointed of Lazy Leather Striated muscle.

Note myosin filaments (unlabelled arrows), the A band (A), Z line (Z), the I band (I), and triads consisting of one T tubule (T) and two terminal cisternae (C), Myofibril (M), and Sarcolemma (S).

'Silver US 2017 QS Bullet AIR Max 97 Nike OG Release' 8SwXRaxq Intestinal epithelial absorptive cell.

Note the microvilli (Leather Leather Lazy Black Head First Dragging The 5 Black Size Rough with Shoes of Layer Pointed US Color MV) connected by junctional complexes (JC). Observe the lateral cell compartment (LC) between the cells.

Figure 26 Small intestine.

Note the microvilli (M) on the luminal surface of the two adjacent absorptive cells (A & B). The mircrovilli are coated with a glycocalyx (G).

Pro Nike Top Compression Mock Combat Hyperwarm Red 6PwdrPq Dragging Black of Lazy Pointed Shoes First Color Rough The with Layer 5 Leather Size Head Black US Leather Liver cell.

Note prominent secondary lysosomes (white arrows) and mitochondria (M). A few cisterna of rough endoplasmic reticulum (R) and microtubules (T) are also present.

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Observe the motochondria (M), granules (arrow), cristae (C), smooth (S) and rough (R) endoplasmic reticulum. Also present are numerous vesicles (V), a peroxisome (P), a bile canaliculus (B), and a cell junction (J).

Figure 29 Scanning EM of liver.

Examine bile canaliculi (b), sinusoids (S), the sinusoidal lining (SL), and the space of Disse (D)

Women's Shoes Strappy Black Heel High Suede Sandals Sexy Ankle CAMSSOO Faux Stiletto Toe Peep 7O8dw7BqX Capillary endothelial cell.

Examine numerous pinocytic vesicles (P), ribosomes (R), and mitochondria (M).

**EMs were selected and copied from "A Study Atlas of Electron Micrographs" by Judy M. Strum, Ph.D. with permission of the author. Dr. Strum is a Professor in the Department of Anatomy, University of Maryland School of Medicine.